Input Sequence

QUERY (1331 aa) 

ATGGCCGAAT CGGACAAAAT AAACATCGAT TCGATCATCC AGCGCMAESD
KINIDSIIQR CTTTTGGAAG TGAAGGGCTC AAGACCTGGT AAAAATGTTC
AGTTALLEVK GSRPGKNVQL ACTGAGCAAG AGATTCGAGG ATTGTGTCTA
AAATCTCGAG AAATCTEQEI RGLCLKSREI TTTCTTAGTC AGCCAATACT
ACTGGAGCTG GAGGCACCCC TCAAGFLSQP ILLELEAPLK ATTTGTGGTG
ATGTTCATGG TCAGTACTAT GACCTGCTCA GGCTTICGDV HGQYYDLLRL
TTTGAGTATG GAGGATATCC ACCAGAGAGT AACTACCTAT TTCTGFEYGG
YPPESNYLFL GGAGACTATG TTGACAGAGG CAAGCAGTCA CTGGAGACCA
TCTGCGDYVD RGKQSLETIC CTGCTGCTTG CTTACAAGAT CAAATATCCA
GAGAACTTCT TTCTTLLLAY KIKYPENFFL CTCAGAGGAA ACCATGAATG
TGCCTCAATC AACAGGATAT ATGGALRGNH ECASINRIYG TTTTATGATG
AATGTAAAAG ACGTTATAAC ATCAAGCTCT GGAAAFYDEC KRRYNIKLWK
ACATTCACAG ACTGTTTCAA CTGCTTGCCA GTAGCTGCTA TTGTGTFTDC
FNCLPVAAIV GATGAAAAGA TTTTTTGTTG TCATGGAGGT CTTTCTCCAG
ACCTGDEKIF CCHGGLSPDL CAATCAATGG AACAGATTCG GCGTGTGATG
AGGCCTACGG ATGTCQSMEQ IRRVMRPTDV CCTGACCAGG GTTTGCTGTG
TGATCTGCTC TGGGCTGACC CTGATPDQGL LCDLLWADPD AAAGATGTCA
TGGGCTGGGG AGAGAATGAT CGTGGTGTTT CCTTCKDVMG WGENDRGVSF
ACCTTCGGGT CTGATGTCGT GGCCAAGTTT TTGCACAAAC ATGACTFGSD
VVAKFLHKHD ATGGATCTCA TATGCAGAGC CCATCAGGTG GTGGAGGATG
GCTACMDLIC RAHQVVEDGY GAGTTCTTTG CGAAGAGGCA GTTAGTCACG
CTTTTCTCTG CTCCCEFFAK RQLVTLFSAP AACTACTGCG GCGAGTTTGA
CAATGCAGGT GCCATGATGA GTGTTNYCGE FDNAGAMMSV GATGAGACCC
TTATGTGCTC ATTTCAGATT CTGAAACCAG CAGAGDETLM CSFQILKPAE
AAGAAGGTGC TGTCATATGG CGGCGCTGGA GGATTTGGGA ACAGCKKVLS
YGGAGGFGNS AGACCTGTAA CTCCTCCTCG GAACACAGCC AAGGGTGGCA
AAGCCRPVTP PRNTAKGGKA AAGAAATAAK K

Results of Subprograms

*** Warning: 1st aa is not methyonine

PSG:  a new signal peptide prediction method
      N-region:  length 0;  pos.chg 0;  neg.chg 0
      H-region:  length 47;  peak value   6.70
      PSG score:   2.30

GvH:  von Heijne's method for signal seq. recognition
      GvH score (threshold: -2.1):  -4.24
      possible cleavage site: between 16 and 17

>>> Seems to have no N-terminal signal peptide

ALOM: Klein et al's method for TM region allocation
      Init position for calculation: 1
      Tentative number of TMS(s) for the threshold  0.5:   4
      Number of TMS(s) for threshold  0.5:   0
      PERIPHERAL  Likelihood =  1.32 (at 1073)
      ALOM score:  -0.75  (number of TMSs: 0)

MTOP: Prediction of membrane topology (Hartmann et al.)
      Center position for calculation: 6
      Charge difference: -2.0   C(-1.0) - N( 1.0)
      N >= C: N-terminal side will be inside

MITDISC: discrimination of mitochondrial targeting seq
      R content:       0       Hyd Moment(75):  1.08
      Hyd Moment(95):  1.04    G content:       9
      D/E content:     1       S/T content:     8
      Score: -8.16

Gavel: prediction of cleavage sites for mitochondrial preseq
      cleavage site motif not found

NUCDISC: discrimination of nuclear localization signals
      pat4: none
      pat7: none
      bipartite: none
      content of basic residues:   3.1%
      NLS Score: -0.47

KDEL: ER retention motif in the C-terminus: none

ER Membrane Retention Signals: 
      KKXX-like motif in the C-terminus: TAAK

SKL: peroxisomal targeting signal in the C-terminus: none

SKL2: 2nd peroxisomal targeting signal:  none

VAC: possible vacuolar targeting motif: none

RNA-binding motif: none

Actinin-type actin-binding motif:
      type 1: none
      type 2: none

NMYR: N-myristoylation pattern : none

Prenylation motif: none

memYQRL: transport motif from cell surface to Golgi: none

Tyrosines in the tail: none

Dileucine motif in the tail: none

checking 63 PROSITE DNA binding motifs:  none

checking 71 PROSITE ribosomal protein motifs:  none

checking 33 PROSITE prokaryotic DNA binding motifs:  none

NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination
      Prediction: cytoplasmic
      Reliability: 89

COIL: Lupas's algorithm to detect coiled-coil regions
      total: 0 residues 

	 60.9 %: cytoplasmic
	 17.4 %: nuclear
	  8.7 %: extracellular, including cell wall
	  4.3 %: endoplasmic reticulum
	  4.3 %: peroxisomal
	  4.3 %: mitochondrial

>> prediction for QUERY is cyt (k=23)